Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology
Article Title: Gamma-aminobutyric acid-salt attenuated high cholesterol/high salt diet induced hypertension in mice
doi: 10.4196/kjpp.2021.25.1.27
Figure Lengend Snippet: (A) The qRT-PCR graph showing the mRNA expressions of GABA B receptor in media, salt or GABA-salt treated mouse vascular smooth muscle cell (MOVAS). (B) Immunofluorescent images showing Endothelin-1 (ET-1) ( green , nuclei; blue ) expressions in media, salt or GABA-salt treated MOVAS. Scale bar = 50 µm. (C, D) Immunohistochemistry staining image and graph showing proliferating cell nuclear antigen (PCNA), as proliferating cell marker, positive cell ( brown , arrows) in hypertensive mice. Scale bar = 50 µm. H&E staining image and graph showing changes of vessel thicknesses in hypertensive mice. Scale bar = 500 µm. *p < 0.05, and **p < 0.01 vs. culture media or normal diet (ND); $ p < 0.05 vs. salt or high cholesterol diet (HCD)/salt.
Article Snippet: Murine vascular smooth muscle cells (MOVAS) were also purchased from the ATCC and grown in DMEM (Hyclone) supplemented with 10% FBS (Merck Millipore) and antibiotics 0.2 mg/ml G-418 (Sigma-Aldrich, Burlington, MA, USA).
Techniques: Quantitative RT-PCR, Immunohistochemistry, Staining, Marker